THE ROLE OF CYTOGENETIC INSTABILITY AND POLYMORPHISM OF GENES OF
GLUTATHIONE-S-TRANSFERASE AND FILAGGRINE PROTEINS IN DEVELOPMENT OF OCCUPATIONAL
DERMATITIS IN OIL FIELD WORKERS 

Il'inskikh Nikolay Nikolaevich, Doctor of biological sciences, professor,  head of sub-department of biology and genetics, Siberian State Medical University (2 Moskovsky trakt, Tomsk, Russia), nauka-tomsk@yandex.ru
Il'inskikh Ekaterina Nikolaevna, Doctor of medical sciences, professor, sub-department of biology and genetics, Siberian State Medical University (2 Moskovsky trakt, Tomsk, Russia), nauka-tomsk@yandex.ru

 616.5-001.1-002-056.3-057-092:575.224.23:622.276

 10.21685/2072-3032-2017-1-2

Background. The authors studied the frequencies of cytogenetic aberrations (micronucleous test) in buccal epithelium cells of oil field workers, who suffered from different forms of allergic occupational dermatitis caused by mutated genes FLG and GSTM1 in their genomes. It is important to introduce new scientifically based criteria for hiring personnel for oil fields in Western Siberia.
Materials and methods. There were examined 209 oil field workers at the age of 21–39 years old. They were under observation at clinics of Tomsk and Tumen due to allergic manifestation of occupational dermatitis. 207 men without allergic occupational dermatitis were regarded as a control group. Genealogical and cytogenetic methods were implemented, as well as the restriction fragment length polymorphism  analysis of PCR products to identify mutated genes GSTM1 and FLG.
Results. It was detected that oil field workers, with genotypes that contained mutated allele GSTM1 (homozygote or heterozygote), had higher concentrations of epithelial cells with micronuclei. It did not depend on FLG gene variants. Among the the examined oil field workers there were no men that would have both mutated alleles of FLG genes (282de14) and homozygous GSTM1 (0/0) (as well as heterozygous GSTM1 (0/+), while among those in the control group there were such people.
Conclusions. The level of mutational rate that was detected during the examination of oil industry workers is the result of a combined effect of different factors on the genome. It should become a base for implementation of new, scientifically based criteria for hiring personnel for oil fields in Western Siberia.

allergic occupational dermatitis, FLG, GSTM1, terms of employment, oilfield of the north of Siberia, mutation, micronucleus test, natural factors

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